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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
15/08/2016 |
Actualizado : |
08/03/2021 |
Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
Autor : |
MACIAS RIOSECO, M.; CAFFARENA, R.; FRAGA, M.; GIANNITTI, F.; BERRUTA WILKINS, Y.; CASAUX, L.; SAUNES, A.; FERNÁNDEZ, L.; BAÑALES, P.; SILVEIRA, C.S.; RIET-CORREA, F. |
Afiliación : |
MELISSA MACIAS RIOSECO, INIA (Instituto Nacional de Investigación Agropecuaria); RUBEN CAFFARENA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; YERBEL BERRUTA WILKINS, Veterinario en Actividad Liberal, Uruguay.; LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria); ALEJANDRA SAUNES, MGAP. (Ministerio de Ganadería Agricultura y Pesca). Dirección de Laboratorios Veterinarios, Uruguay.; LEANDRO FERNANDEZ, MGAP. (Ministerio de Ganadería Agricultura y Pesca). Dirección de Laboratorios Veterinarios, Uruguay.; PEDRO BAÑALES, MGAP. (Ministerio de Ganadería Agricultura y Pesca). Dirección de Laboratorios Veterinarios, Uruguay.; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Campylobacter fetus subsp. venerealis y presunta neosporosis como causa de abortos en un rodeo lechero de Uruguay con altos títulos de anticuerpos para Leptospira interrogans serovares pomona, hardjo-prajitno, wolffi y hardjo-bovins. |
Fecha de publicación : |
2016 |
Fuente / Imprenta : |
In: Jornadas Uruguayas de Buiatría, 44., 2016, Paysandú, UY.; Gianneechini, E.; Elizondo, V. (Ed.).Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2016. |
Páginas : |
p. 248-251 |
Idioma : |
Español |
Palabras claves : |
CULTIVO BACTERIANO; INMUNOFLUORESCENCIA DIRECTA (IFD); NECROPSIS; PLATAFORMA DE SALUD ANIMAL; SECUENCIACIÓN DEL ADNr. |
Thesagro : |
ENFERMEDADES DE LOS ANIMALES. |
Asunto categoría : |
L73 Enfermedades de los animales |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/15290/1/JB2016-248-251.pdf
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Marc : |
LEADER 01200naa a2200313 a 4500 001 1055268 005 2021-03-08 008 2016 bl uuuu u00u1 u #d 100 1 $aMACIAS RIOSECO, M. 245 $aCampylobacter fetus subsp. venerealis y presunta neosporosis como causa de abortos en un rodeo lechero de Uruguay con altos títulos de anticuerpos para Leptospira interrogans serovares pomona, hardjo-prajitno, wolffi y hardjo-bovins. 260 $c2016 300 $ap. 248-251 650 $aENFERMEDADES DE LOS ANIMALES 653 $aCULTIVO BACTERIANO 653 $aINMUNOFLUORESCENCIA DIRECTA (IFD) 653 $aNECROPSIS 653 $aPLATAFORMA DE SALUD ANIMAL 653 $aSECUENCIACIÓN DEL ADNr 700 1 $aCAFFARENA, R. 700 1 $aFRAGA, M. 700 1 $aGIANNITTI, F. 700 1 $aBERRUTA WILKINS, Y. 700 1 $aCASAUX, L. 700 1 $aSAUNES, A. 700 1 $aFERNÁNDEZ, L. 700 1 $aBAÑALES, P. 700 1 $aSILVEIRA, C.S. 700 1 $aRIET-CORREA, F. 773 $tIn: Jornadas Uruguayas de Buiatría, 44., 2016, Paysandú, UY.; Gianneechini, E.; Elizondo, V. (Ed.).Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2016.
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
24/10/2014 |
Actualizado : |
15/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
B - 4 |
Autor : |
ALEM, D.; DIAZ, P.; LEONI, C.; DE SIMONE, S.G.; CORREA, A.; OPPEZZO, P.; DALLA RIZZA, M. |
Afiliación : |
DIEGO MARTIN ALEM GLISON, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; PAOLA ALEXANDRA DIAZ DELLAVALLE, Departamento de Ciencia y Tecnología de los Alimentos, Facultad de Ingeniería y Tecnologías, Universidad Católica del Uruguay (UCU), Montevideo, Uruguay; CAROLINA LEONI VELAZCO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
In Search of Topical Agricultural Biofungicides: Properties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis. |
Fecha de publicación : |
2014 |
Fuente / Imprenta : |
Journal of Microbial & Biochemical Technology, 2014, v. 6, no.5, p. 268-273. |
ISSN : |
1948-5948 |
DOI : |
10.4172/1948-5948.1000155 |
Idioma : |
Inglés |
Contenido : |
ABSTRACT.
Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an
interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP
was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For
topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easy to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially in relation to molecule bioavailability. MenosABSTRACT.
Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an
interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP
was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For
topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resista... Presentar Todo |
Thesagro : |
ACTIVIDAD ANTIMICROBIANA; ALTERNARIA; BIOTECNOLOGÍA; PÉPTIDOS. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/3627/1/Dalla-Rizza-M.-2014.-Jr.Microb.Biochem.-Technol.-v.65.pdf
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Marc : |
LEADER 02624naa a2200265 a 4500 001 1051279 005 2019-10-15 008 2014 bl uuuu u00u1 u #d 022 $a1948-5948 024 7 $a10.4172/1948-5948.1000155$2DOI 100 1 $aALEM, D. 245 $aIn Search of Topical Agricultural Biofungicides$bProperties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis.$h[electronic resource] 260 $c2014 520 $aABSTRACT. Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easy to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially in relation to molecule bioavailability. 650 $aACTIVIDAD ANTIMICROBIANA 650 $aALTERNARIA 650 $aBIOTECNOLOGÍA 650 $aPÉPTIDOS 700 1 $aDIAZ, P. 700 1 $aLEONI, C. 700 1 $aDE SIMONE, S.G. 700 1 $aCORREA, A. 700 1 $aOPPEZZO, P. 700 1 $aDALLA RIZZA, M. 773 $tJournal of Microbial & Biochemical Technology, 2014$gv. 6, no.5, p. 268-273.
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